A Secret Weapon For flow cytometers
A Secret Weapon For flow cytometers
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Data-enriched characterization of cell populations. Morphological facts from photographs can insert for the richness of flow cytometry knowledge. As an example, the figure reveals an otherwise regular apoptosis assay working with Annexin V and PI, adding mobile imaging to characterize cells in Every population to expose morphologically distinctive features. These insights couldn't are actually obtained from multiplex staining by itself.
Regardless of whether you’re a starter or a specialist, examine our means to progress your research and experience in flow cytometry.
(B) RAW 264.7 macrophages are often annotated inaccurately with regard to particle rely because of their complexity and noncircular borders. The amount of singlets accessible for Assessment boosts as soon as the consumer trains and employs a more correct model.
Uncover new insights into your experimental information by visualizing populations working with combined fluorescence and morphology data. Our software package interface wizards allow downsampling, concatenation (merged documents) and dimensionality applications that are designed in the program for an intuitive, streamlined information analysis experience.
"A picture isn't really rather worthy of a thousand dots, nonetheless it seriously helps to see photos. It is great to view photos that correlate with staining. As an example, AnnexinV-beneficial cells that are morphologically diverse than healthy cells, or CD14+ monocytes which are much larger and much more textured than CD3+/CD4+/CD14- lymphocytes.
Here, a highly skilled consumer has gated singlets confidently. Right after evaluating the guide singlet gate, the CytPix impression-derived parameter ParticleCount reveals this gate is made up of greater than 4% aggregates.
Optimizing gating method. Even sturdy guide singlet gating is mistake-vulnerable and continues to be a subjective decision place in Virtually all flow cytometry assays. Imaging may be used to confirm and alter gates to include only one cells of curiosity.
Our scientists will structure panels to suit your needs as over and over you may need—cost-free, no merchandise buy essential.
The Attune NxT cytometer will be the trustworthy workhorse instrument your lab can depend on for dependable and trusted performance. The Attune CytPix model contains a high-velocity brightfield digital camera and AI-driven picture Investigation program, with new updates such as equipment Discovering tools and an intuitive person interface, enabling Improved information precision, precision, and speedier insights into sample biology.
Users can see event illustrations or photos in true-time to verify sample quality, improve protocols, and document the morphology of every mobile populace identified for Examination. Imaging capture also benefits from acoustic focusing, which helps you to placement the cells so that a sharp, centered graphic is attained.
AI-pushed automated graphic Investigation with the Attune Cytometric Software program translates function characteristics into distinctive morphology parameters that may be coupled with typical fluorescence and scatter parameters to reveal really reproducible inhabitants variations and exceptional subsets.
Software Take note: Automated graphic Assessment lowers person-to-consumer variability in flow cytometry gating approaches
Built-in dimensionality reduction equipment greater resolution into populations. Fluorescently gated populations back-gated onto UMAP plot read more to verify envisioned populace resolution in lysed entire human blood. Person can watch occasions from Each individual cluster for Visible confirmation of cell phenotypes.
Versatile payment strategy from Agilent Economical Options allow you to have the significant instrumentation you may need for fast job, or to improve your lab.
At the heart of each Attune Flow Cytometer lies our revolutionary acoustic focusing engineering, which utilizes seem waves to meticulously align cells within the flow mobile. This modern strategy surpasses traditional hydrodynamic procedures, resulting in faster processing speeds, exceptional info excellent, and increased sample throughput.
Because the mobile line experienced Formerly been analyzed within the Attune CytPix Flow Cytometer, the scientists went again to the pictures and ended up capable of document the microbial infection at the very least 5 times earlier.
The Attune CytPix Flow Cytometer allows customers to visualize samples for the duration of acquisition, validate the precision of gating approaches, and guidance outcomes with trusted documentation for publication uses.
Below, we show that by gating techniques employing impression-primarily based quantitative parameters can distinguish interacting cells from coincident functions to much more precisely review interacting cells.